Multi-Channel & Color Analysis

Step 4.1 — Compose pseudo-color overlays from multiple images; co-localization (Pearson/Manders), channel ratio and difference maps
Upload 1–4 single-channel images (PNG, JPG, TIFF). Each image is treated as one fluorescence channel or spectral band.

Configure channels below and click Compose.

Compose Options

Each channel is normalised 0–1 before colour mapping. Overlapping colours mix additively.