Biological Quantification & Morphometrics

Step 5.1 — Detailed cell/organelle measurements: diameter, perimeter, volume, MFI per channel; co-localization scatter plot; CT HU density calibration

Upload Image Upload a grayscale, multi-channel TIFF, or DICOM CT image to begin.

Detection Settings

Threshold %ile

Min area (px)

Max objects

Smooth σ

CT HU Calibration

Optional. If a CT image is loaded, enter the expected HU of the dominant tissue to calibrate density.

Expected HU

Co-localization Scatter

Requires a multi-channel TIFF. Select two channels to compare.

Channel 1 (x)

Channel 2 (y)